TESSEK Separon SGX - unmodified silica for adsorption chromatography of low molecular weight solutes in a normal phase mode. Typical applications include separation of aromatic and polyaromatic hydrocarbons, lipids, steroids, fat soluble vitamins, glycoside derivatives, drugs and pharmaceuticals.

TESSEK Separon SGX CN and SGX NH₂ - are two medium polar-bonded phases for separation of low molecular weight solutes in normal and reversed phase mode. The bonded phases offer higher reproducibility of retention volumes and shorter equilibration times compared to unmodified silica. The TESSEK Separon SGX CN contains cyanoethyl groups and TESSEK Separon SGX NH₂ bonded aminopropyl groups. Typical applications include separations of aromatic hydrocarbons and their derivatives, pesticides, organophosphates, fat soluble vitamins, drugs and pharmaceuticals. The TESSEK Separon SGX NH₂ is suited for separation of mono and di-saccharides in acetonitrile-water eluents also.

Reversed Phase Chromatography

TESSEK Separon SGX C8, SGX C18, SGX RPS, SGX Phenyl - are reversed phase sorbents differing in functionality and carbon loading. TESSEK Separon SGX C18 is octadecyl modified, the most commonly used sorbent with widest range of applications including pesticides, amino acid derivatives, peptides, catecholamines, drugs, pharmaceuticals, steroids, antibiotics, organic acids, nucleotides and many others. TESSEK Separon SGX RPS is octadecyl modified with very high carbon content. It offers higher retention in comparison to SGX C18 and is especially suited for ion pair chromatography and separation of oligosaccharides and other polar compounds in highly aqueous eluents. Due to its polymeric character it offers good selectivity for polyaromatic hydrocarbons. TESSEK Separon SGX C8 is an octyl modified sorbent, which offers lower retention and changed selectivity, advantageous for separation of catecholamines, peptides and some pharmaceuticals. TESSEK Separon SGX Phenyl with bonded phenyl groups offers lower retention and changed selectivity useful for polycondensed aromatic hydrocarbons, polar aromatic compounds and unsaturated fatty acids. For separation of basic compounds the use of mobile phase modifiers (e.g. triethylamine) or low pH eluents is recommended.

Ion Exchange Chromatography

TESSEK Separon SGX CX is a strongly acidic cation exchanger with a benzenesulfonic acid functional group and TESSEK Separon SGX AX is a strongly basic anion exchanger with aliphatic quaternary amine functional group, suited for ion exchange chromatography of ionic compounds both in aqueous and organic eluents.

Macroporous Silica Sorbents

Based on the TESSEK Separon SGX, those sorbents are prepared by special treatment to provide material with large pores possessing a narrow pore size distribution.

TESSEK Separon SGX 200 - is prepared by hydrothermal treatment and is intended for size exclusion chromatography of organic solvent soluble polymers in the molecular weight range 1 - 100 kDa.

TESSEK Separon SGX 500 and 1000 are two macroporous silicas for size exclusion chromatography of organic solvent soluble polymers in the molecular weight ranges 10 - 1 000 kDa and 50 - 3 000 kDa respectively.

Ordering Information and Specifications

Rem.: Based on mercury porosimetry

Sorbent Functional Group

• SGX C18 - octadecyl (approx. 18 % carbon)
  
• SGX CN - cyanoethyl (approx. 9 % carbon, 1.4 % nitrogen)
  
• SGX NH₂ - aminopropyl (approx. 6.5 % carbon, 2.2 % nitrogen)
  
• SGX RPS - octadecyl (approx. 24 % carbon)
  
• SGX CX - benzenesulfoc (approx. 15 % carbon, 0.6 mmol/g)
  
• SGX AX - quart. amine (approx. 16 % carbon, 0.5 mmol/g)
  
• SGX Phenyl - phenyl (approx. 15 % carbon)
  
• SGX C8 - octyl (approx. 12 % carbon)
  

Bulk Sorbents TESSEK Separon SGX are available in 10 g, 100 g and bulk quantities.

The sorbents in 60 µm particle size are available also in SILICA-cart cartridges for solid phase extraction. When ordering, use Cat.No. 421. for cartridges plus Cat. No. of sorbent packed.

Packed Columns

When ordering packed columns, use first three digit Cat. No. to indicate the type of column plus Cat. No. of sorbent used.

Stainless steel columns are available in two diameters and different lengths. 4 mm ID columns are available packed with all SGX sorbents in 5, 7, and 10 µm particle size, 8 mm ID columns preferrably with 7 µm particle size.

Titanium zirconium columns are available in 8 mm diameter and different lengths. They are available packed with all SGX sorbents in 7 µm particle size.

CGC columns 3.3 mm ID are available packed with all sorbents in 5, 7, and 10 µm particle size, CGC microcolumns 1 mm I.D. with 5 µm particle size only.