TESSEK stainless steel hplc columns packed with TESSEK Separon HEMA hydroxyethyl methacrylate and TESSEK Separon SGX silica sorbents for analytical and semi-preparative purposes. The modern design of hplc columns ensures low dead volumes and compatibility of the hplc columns with principally all types of chromatographic instruments. The exchangeable coupling fittings enable coupling with 1.5 mm or 1/16" OD capillaries. Either M5 or UNF 10-32 male thread bushings can be used. The hplc columns are delivered single-packed with an individual test report and equipped with the UNF 10-32 coupling fittings. (When ordering M5 coupling fittings, please specify this in your order).
The 4 mm ID x 250 mm column format is still the most commonly used for analytical purposes. The 8 mm ID x 250 mm columns are especially suited for semi-preparative separations and size exclusion chromatography. The 8 mm ID x 80 mm column format is well suited for work with polymer based sorbents for analytical and semipreparative purposes with gradient elution.
In spacial cases, the commonly use stainless steel is not inert enough. When aggresive solvents are used or metal sensitive proteins are to be separated, inert materials must be used thorough all the chromatographic systems. The Titanium Zirconium alloy offers high inertnes combined with high mechanical strength. The 8x250 mm columns are well suited for semipreparative and size exclusion separations, while the 8x80 mm format is ideal for gradient elutions. All column parts are machined from titanium-zirconium alloy. Ceramic paper, PTFE sieves and PTFE sealing rings are used to support the sorbent. The column material is resistant to most eluents including halide or low pH organic acids containing solvents. It may be attacked by hydrogenfluoric acid.
All column parts are metal-free and made of PEEK (poly-ether-ether-ketone). The column will stand max 90 bar continuously and is excellently suited for all common HPLC and LC solvents. However, use of concentrated nitric and sulfuric acids, tetrahydrofuran and dimethylsulfoxide is not recommended. The column dimensions include 4.6 ID times 30, 50, 100, 150 and 250 mm lengths, as well as 7.5 ID times 50, 75, 250 and 300 mm lengths.
The TESSEK-patented design of compact glass cartridge offers the unique combination of a low-cost and high-performance HPLC column. The CGC columns exhibit high pressure resistance (up to 30 MPa), total inertness, chemical resistance, high efficiency and, due to the smaller diameter, increased sensitivity. Moreover, any damage to the column bed is instantly visible. The columns are closed with fine mesh stainless steel sieves (silica based sorbents) or by inert teflon-ceramic paper combination (HEMA sorbents)
Three types of CGC cartridges packed with TESSEK Separon SGX and HEMA sorbents are currently available:
Microbore columns (0.5-2mm ID) offer greater mass sensitivity and low solvent consumption in comparison to standard analytical columns. They are indispensable when the sample size is limited or when expensive, rare or highly hazardous solvents are used. Best results are obtained with specially constructed micro chromatographs. However, standard equipment can be used if low volume injectors and detector cells (below 1 µl) are used and dead volumes are eliminated. The pump must be capable to deliver solvent at flow rates from 10 µl/min.
Two types of CGC microbore columns packed with TESSEK Separon SGX sorbents are currently available:
CGC Column Holders
For operating the CGC cartridges, an appropriate column holder must be used. TESSEK offers new column holder sets, completely machined from stainless steel. For coupling to the instrument, the 1.5 mm or 1/16" OD capillaries can be used. These sets are supplied in two variants:
The following sets are available:
These sets allows the operation of a single CGC column of specified length. Each of those sets contains one holder body, two screw caps and two coupling fittings.
These sets are intended for the operation of coupled CGC columns, especially for CGC 3x30 guard column coupling. They can also be used for the operation of a single column. In combination with an other column holder body they can serve for the operation of any combination of CGC columns. Each of these sets contains two holder bodies of specified lengths, two screw caps, two column fittings, one column coupling union and one holder body connector.
For the coupling of CGC 1x150 micro-columns special narrow bore coupling unions are recommended. The connecting capillaries should be of 0.1 or 0.2 mm ID and as short as possible.
|CGC Column Holder||Cat. No.|
|CGC column holder 30 mm M5||900 001011|
|CGC column holder 30 mm UNF||900 001021|
|CGC column holder 70 mm M5||900 001013|
|CGC column holder 70 mm UNF||900 001023|
|CGC column holder 150 mm M5||900 001017|
|CGC column holder 150 mm UNF||900 001027|
|CGC column holder 70+30 mm M5||900 001015|
|CGC column holder 70+30 mm UNF||900 001025|
|CGC column holder 150+30 mm M5||900 001019|
|CGC column holder 150+30 mm UNF||900 001029|
Besides analytical applications, HPLC chromatography
is an indispensable tool also for preparation of small quantities
of purified compounds. For laboratory scale purifications, TESSEK
offers preparative columns, enabling to purify tenth to hundreds
milligrams of compounds in one run. The columns are manufactured
from 316 grade stainless steel, which guarantees an excellent
chemical inertness and corrosion stability. The columns are available
packed with whole range of TESSEK Separon SGX or TESSEK Separon
HEMA sorbents. In 25x250 mm format, fixed or hand tight endfitings
are available. In the 46x300 mm format, only hand tight fittings
are available. Other column lengths can be prepared on request.
The SILICA-cart and HEMA-cart system is specially designed to optimize sample preparation and storage. The cartridge works by extracting certain classes of compounds from injected samples while allowing others to pass through. The system can be used to concentrate trace components from environmental and biological samples. It is ideal for the preparation of samples for analysis by HPLC, GC, TLC, RIA and other methods. The cartridge is terminated with a male and female standard syringe cone (Luer) and can be used with a syringe, a peristaltic pump or a suitable vacuum-operated manifold. The maximum operating pressure is 0.3 MPa; the suction mode operation is recommended. The cartridges are supplied with tight caps to ensure long-term sample storage.
The SILICA-cart system consists of a prepacked 1 ml polypropylene cartridge (medical grade Tatren PD 140) packed with 60 µm spherical silica TESSEK Separon SGX sorbents.
The HEMA-cart system consists of a pre-packed 1 ml polypropylene cartridge (medical grade Tatren PD 140) packed with 60 µm TESSEK Separon HEMA-BIO 1000 modified sorbents.
|Product||Colour Code||Cat. No.|
|SILICA-cart SGX||yellow-yellow||421 310003|
|SILICA-cart C18||orange-orange||421 310013|
|SILICA-cart CN||yellow-orange||421 310023|
|SILICA-cart NH2||yellow-brown||421 310033|
|SILICA-cart RPS||orange-black||421 310043|
|SILICA-cart C8||yellow-black||421 310083|
|SILICA-cart CX||green-orange||421 310053|
|SILICA-cart AX||blue-orange||421 310063|
|SILICA-cart Phenyl||orange-grey||421 310073|
|HEMA-cart DEAE||blue-blue||421 172043|
|HEMA-cart CM||green-green||421 172053|
|HEMA-cart Q||blue-black||421 172093|
|HEMA-cart SB||green-black||421 172133|
|HEMA-cart E||grey-grey||421 172013|
|HEMA-cart E-L||black-black||421 172023|
|HEMA-cart E-H||black-red||421 172083|
|HEMA-cart VS-L||grey-red||421 172113|
|HEMA-cart VS||black-grey||421 172123|
|HEMA-cart C18||yellow-black||421 170153|
|HEMA-cart rProtA||labeled||421 172983|